Chromatography Techniques Tutorial
- Chromatography is an analytical technique used to determine the purity of a substance or to separate a mixture into its components.
- The stationary phase refers to the solid or liquid to which components in a mixture bind or adsorb.
- The mobile phase refers to the liquid or gas that moves the components in a mixture over the stationary phase.
- Components in a mixture are separated based on their different abilities to bind or adsorb to the stationary phase, and on their different abilities to desorb or dissolve in the mobile phase.
- In paper chromatography and thin layer chromatography the retardation factor, Rf, is a comparison of the distance travelled by a component to the distance travelled by the solvent.
- In gas chromatography and high-performance liquid chromatography, the time retained in the column, the retention time (Rt), is measured.