Mass Spectroscopy for Structural Determination
- In a Mass Spectrometer, a molecule is bombarded with a high-energy electron beam causing it to fragment into smaller ions.
- Each of these fragments has its own molecular mass.
- Mass Spectrometers use the difference in mass-to-charge ratio (m/z or m/e) of ionised atoms and molecules to separate them from each other.
- A mass spectrum is the record of the quantity of ions of particular mass-to-charge ratio.
- Peak heights on a mass spectrum are proportional to the number of ions of each mass.
- Mass Spectroscopy can be used to determine:
(b) molecular mass of a molecule
(c) structure of a molecule