- Electrophoresis is a separation technique based on the mobility of ions in an electric field.
- Electrophoresis can be used to separate mixtures of amino acids or fragments of DNA.
- The mobility of ions in an electric field depends on:
Positively charged ions moved towards the negatively charged electrode.
Negatively charged ions moved towards the positively charged electrode.
Neutral particles will remain stationary.
Ions with the least mass will move further than ions with greater mass.
- DNA fragments are negatively charged due to the negatively charged phosphate groups making up the backbone of the molecule.
- Amino acids can be neutral, acidic, or basic, depending on the pH of the solution.