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A Summary of Spectroscopy Techniques

Type Features Uses
Flame Tests
  • inexpensive

  • fast, but some colours may be masked by others making it unreliable

  • qualitative technique

  • limited application as many common metals (e.g., Al & Mg) do not colour a flame

  • a type of emission spectroscopy which can be observed by the naked eye or by using a spectroscope to see a series of bright lines
Identification of metals in some salts

  • Li+       crimson

  • Na+     yellow

  • K+       lilac

  • Ca2+   brick red

  • Sr2+   scarlet

  • Ba2+   green

  • Cu2+   green-blue

Colorimetry
  • relatively inexpensive

  • fast, requiring a set of standard solutions

  • qualitative & quantitative

  • a type of absorption spectroscopy limited to coloured solutions or compounds
  • copper (II) sulfate in garden sprays

  • iron (III) concentration using KSCN to form the coloured Fe(CNS)2+ complex

  • coloured solutions such as food colourings
wavelength of light is selected for maximum absorbance & a calibration curve is constructed of absorbance versus concentration

Atomic Absorption Spectroscopy (AAS)
  • expensive

  • fast, requiring a set of standard solutions

  • quantitative

  • a type of absorption spectroscopy that can be used for all metals and metalloids (semi-metals)
  • mercury in shellfish

  • lead in soil samples

  • trace metals in mineral waters
correct hollow cathode tube selected & a calibration curve constructed of absorbance versus concentration. (solutions may have to be diluted)

UV-visible spectroscopy
  • expensive

  • fast, requiring a set of standard solutions

  • qualitative & quantitative

  • a type of absorption spectroscopy limited to coloured solutions or compounds
  • concentration of some coloured pharmaceuticals in blood serum

  • concentration of metal ions using coloured complexes

  • concentration of food colourings
wavelength of light selected for maximum absorbance and a calibration curve constructed of absorbance versus concentration
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Related AUS-e-TUTE Topics

Infrared Spectroscopy

Mass Spectroscopy

Chromatography

 

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